Purification and Serology of an Isolate of Zucchini Yellow Mosaic Virus
Other Title
矮南瓜黃化嵌紋病毒之純化與血清學特性之研究
Journal
中華農業研究
Journal Volume
35
Journal Issue
4
Pages
495-503
Date Issued
1986-12-09
Date
1986-12-09
Author(s)
C.H. Huang
Shiow-Huey Hseu
Y.J. Chao
黃秋雄
許秀惠
趙玉珍
Abstract
An isolate of zucchini yellow mosaic virus, ZYMV-7, was purified and its antiserum produced. Systemic leaves from inoculated zucchini plants taken 9-10 days after inoculation had the highest virus concentration, and were used in virus purification. Yields of purified virus estimated by spectrophotometer were 18-22 mg/l00g leaf tissues. The O. D. ratio of A260/280 from several separate preparations ranged from 1.27 to 1.31. The purified virus preparation obtained from the first isopycnic centrifugation in cesium sulfate was found contaminated with host proteins, however, the contaminated materials could be removed by a second isopycnic centrifugation. The reciprocal titers of antiserum against homologous antigens in crude leaf extracts and purified virus preparations were 2048 and 4096, respectively, in ring interface precipitin tests. 將Gonsalves及Ishii純化木瓜輪點病毒所使用之等密度平衡離心法(Zucchini yellow mosaic virus, ZYMV)略加修改，可成功地純化矮南瓜黃化嵌紋病毒(zucchini yellow mosaic virus, ZYMV)。以接種ZYMV-No.7分離株之矮南瓜爲純化材料，估計每百克病葉可獲得22毫克之純化病毒。紫外光吸收比值A260/280約在1.27至1.31之間。所純化之病毒懸浮液適量混合輔助劑(adjvuant)後曲于入小白兔使產生抗血清。利用界面環沈澱法，將抗血清與本病毒之粗汁液及純化之病毒反應，測得血清力價分別爲2048及4096。
Publisher
農業試驗所
Type
journal article
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