RFLP標誌在果樹育種與品系鑑別的應用
Other Title
The application of RFLP markers in fruit tree breeding and variety identification
Journal
果樹育種研習會專刊
Part Of
農業試驗所特刊第37號
Pages
139-146
ISBN
957-00-2562-X
Date Issued
1993-06
Date
1993-06
Author(s)
劉邦基
Pan-Chi Liou
Abstract
RFLP ( Restriction Fragment Length Polyoorphism）是『限制段的長度多形性』的英文簡稱，它是一種直接利用生物核酸分子序列變異性來探討生物遺傳現象的方法，同時也是一種分子標誌(mo1ecular marker)。此一技術首次建立於1980年，隨即應用在人類的遺傳研究，目前其應用範圍已遍及多種動植、真菌和細菌之遺傳研究上。RFLP具有多項特性，咸認可用來克服木本植物之遺傳研究的某些瓶頸與困難，因而在果樹育種和遺傳研究上極具應用潛力，其主要用途包括：縮短育種時問、保障育種家的權益、和有助於基因轉移之進行等。
RFLP (Restriction Fragment Length Polymorphism) is a relatively new category of genetic marker which allows direct detection of variation at the DNA level. This technology was first developed in 1980 for human genetic study, and has been applied in animal, plant, fungus, and bacteria genetic. RFLP shows some advantages when used in genetic studies, such as co-dominant, simple Mendelian genetic fashion, not tissue or developmental stage specific, and no epistatic interaction. Based on these advantages, it is believed being very useful in woody plant research, and being a powerful tool for enhancing the breeding efficiency in fruit trees. The purpose of this article is to describe some basic techniques in RFLP studies. The potential of using this powerful tool in fruit breeding and some other application have also been discussed.
Event(s)
果樹育種研習會
Subjects
果樹
育種
品系鑑定
RFLP
Frult trees
breeding
varlety identlflcatlon
RFLP
Publisher
農業試驗所
Type
conference paper
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