利用懸浮培養茶葉細胞誘導兒茶素類生合成及其應用性研究

陳英玲; 蔡志賢

利用懸浮培養茶葉細胞誘導兒茶素類生合成及其應用性研究

Other Title

Studies of Formation and Application of Catechins Induced by Mass of Callus from Stem Disc Explants of Tea

Journal

1997特用作物試驗成果研討會專刊

Part Of

農業試驗所特刊第74號

Pages

280-280

ISBN

957-02-1964-5

Date Issued

1997-08

Date

1997-08

Author(s)

陳英玲

蔡志賢

URI

https://scholars.tari.gov.tw/handle/123456789/2906

Abstract

茶樹各品種之莖段培植體在auxin與cytokinins組合之MS培養基中經一個月，可見到癒合組織的發生。青心烏龍莖段培植體癒合組織誘導之最適培養基為NAA 3mg/1組合BA smg/1處理，有80％癒合組織之誘導率。台茶12號莖段培植體癒合組織之誘導以NAA 3mg/l組合BA lmg/l處理時，誘導率最高為80％。台茶13號莖段培植體癒合組織之誘導在2, 4D及BA各5mg/l之處理時得到較高之誘導率60％。台茶12號之懸浮培養細胞培養基為NAA 5mg/l組合BA 1mg/1之MS液體培養基，依生長曲線間隔10-14天需繼代培養，而癒合組織仍具有合成兒茶素之能力。台茶12、13、17號及青心烏龍等品種之莖段培植體所誘導之癒合組織，在含NAA有l-5 mg/l之MS培養基中培養，會有不定根之發生。 Mass of callus were induced from stem disc explants of four tested tea vadeties on MS medium supplemented with auxin and cytokinins for one month. The best calli induction medium for stem disc explants was -MS medium containing 3 mg/l NAA and 5 mg/l BA(80% induced rate) in “Chin-Shin Oolong”, 3mg/l NAA and 1mg/l BA (80% induced rate) in “TTES No.12”, 5mg/l 2,4D and 5mg/1 BA (60% induced rate) in “TTES No.13”. The “TTES No.12” calli was suspension cultured on MS liquid medium containing 5mg/l NAA and 1mg/l BA, based on growth curve, subcultured after 10-14 days. Stem disc of “TTES No.12”, “TTES No.13”, “TTES No.17” and “Chin-Shin Oolong” cultured on MS medium with 1-5 mg/l NAA produced adventitive roots.

Event(s)

1997特用作物試驗成果研討會

Proceedings of A Symposium on the Experimental Results of Special Crops 1997

Publisher

農業試驗所

Type

conference paper

File(s)

Name

publication_no74-36.pdf

Size

97.15 KB

Format

Adobe PDF

Checksum

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