Rice GA3ox1 modulates pollen starch granule accumulation and pollen wall development
Journal
PLOS ONE
Journal Volume
18
Journal Issue
10
Pages
e0292400
Date Issued
2023-10
Author(s)
Kun-Ting Hsieh
Chi-Chih Wu
Shih-Jie Lee
Yu-Heng Chen
Shiau-Yu Shiue
Yi-Chun Liao
Su-Hui Liu
Wen-Hsiung Li
Chang-Sheng Wang
Liang-Jwu Chen
Abstract
The rice GA biosynthetic gene OsGA3ox1 has been proposed to regulate pollen development through the gametophytic manner, but cellular characterization of its mutant pollen is lacking. In this study, three heterozygotic biallelic variants, "-3/-19", "-3/-2" and "-3/-10", each containing one null and one 3bp-deletion allele, were obtained by the CRISPR/Cas9 technique for the functional study of OsGA3ox1. The three homozygotes, "-19/-19", "-2/-2" and "-10/-10", derived from heterozygotic variants, did not affect the development of most vegetative and floral organs but showed a significant reduction in seed-setting rate and in pollen viability. Anatomic characterizations of these mutated osga3ox1 pollens revealed defects in starch granule accumulation and pollen wall development. Additional molecular characterization suggests that abnormal pollen development in the osga3ox1 mutants might be linked to the regulation of transcription factors OsGAMYB, OsTDR and OsbHLH142 during late pollen development. In brief, the rice GA3ox1 is a crucial gene that modulates pollen starch granule accumulation and pollen wall development at the gametophytic phase.
Publisher
Public Library Science
Description
We thank the Division of Biotechnology, TARI, for providing the greenhouse and isolated field to grow the transgenic rice plants.
Type
journal article