Influence of ventilation closures on plant growth parameters, acclimation and anatomy of leaf surface in Scrophularia yoshimurae Yamazaki - a medicinal plant native to Taiwan

Uei-Chern Chen; Chi-Ni Hsia; Dinesh Chandra Agrawal; Hsin-Sheng Tsay; 陳威臣; 夏奇鈮; 蔡新聲

Influence of ventilation closures on plant growth parameters, acclimation and anatomy of leaf surface in Scrophularia yoshimurae Yamazaki - a medicinal plant native to Taiwan

Other Title

透氣性培養容器封口對臺灣本土藥用植物—臺灣雙鋸齒葉玄參組培苗生長、馴化及葉表構造之影響

Journal

Botanical Studies

Journal Volume

47

Journal Issue

3

Pages

259-266

Date Issued

2006-07

Date

2006-07

Author(s)

Uei-Chern Chen

Chi-Ni Hsia

Dinesh Chandra Agrawal

Hsin-Sheng Tsay

陳威臣

夏奇鈮

Dinesh Chandra Agrawal

蔡新聲

URI

https://scholars.tari.gov.tw/handle/123456789/14345

https://ejournal.sinica.edu.tw/bbas/toc/issue-2006-3.html

Abstract

Different ventilation closures, including aluminum foil (AF) and a varying number of dispense papers (DP) had different effects on leaf growth parameters, in vitro rooting, survival rate and the anatomical features of the leaf surface of in vitro and ex vitro acclimated plants of Scrophularia yoshimurae—an important medicinal plant. The lowest plant growth parameters and ex vitro acclimation rate (<7.0%) were obtained using AF as ventilation closure. A scanning electron microscopy (SEM) study of leaf surfaces of plants derived from different ventilation closure treatments showed that parameters—including density and size of epidermal cell and stomata, size of guard cells, and stomata aperture-differed significantly among various treatments, and this in turn affected plant survival rate. Leaves derived from AF treatment had higher epidermal cell (15094 cells/mm2) and stomata (38/mm2) densities than DP treatments. Well-ventilated container closures, such as with DP, improved the morphological characteristics of leaves and in turn enhanced the survival rate during ex vitro acclimation (maximum rate being 66.7%). The present study not only provides an improved micropropagation method of S. yoshimurae but also gives scientific reasons for the different acclimation rates obtained with various container closures.

Subjects

Hyperhydricity

In vitro culture

Scanning electron microscopy

Scrophularia yoshimurae

Stomata

Ventilation closure

Publisher

Springer

Type

journal article

File(s)

Name

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Format

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