使用即時定量RT-PCR 於瓜類褪綠黃化病毒檢驗之評估

Other Title
Evaluation of a TaqMan One-Step Real-Time RT-PCR Method for the Detection of CCYV
Resource
台灣農業研究: 64(3): 204-212
Journal of Taiwan Agricultural Research: 64(3): 204-212
Journal
台灣農業研究
Journal Volume
64
Journal Issue
3
Pages
204-212
Date Issued
2015-09-05
Date
2015-09-05
Author(s)
林育昌
鄧汀欽 
Yu-Chang Lin
Ting-Ching Deng
DOI
URI
Abstract
本研究依據瓜類褪綠黃化病毒（Cucurbit chlorotic yellows virus; CCYV）之鞘蛋白（coat protein）基因設計專一性引子對，並利用real-time RT-PCR與TaqMan螢光探針，建立瓜類褪綠黃化病毒的檢測技術，同時進行田間洋香瓜和南瓜是否感染CCYV之測試。 In this study, a diagnostic assay for Cucurbit chlorotic yellows virus (CCYV) using TaqManbased real-time reverse transcription polymerase chain reaction (qRT-PCR) was developed. Specific primers were designed by targeting the coat protein gene of CCYV.
Subjects
瓜類褪綠黃化病毒
分子偵測
及時反轉錄聚合酶鏈鎖反應
Cucurbit chlorotic yellows virus
Detection
Real-time RT-PCR
Publisher
農業試驗所
Type
journal article
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