萵苣嵌紋病毒之多元抗血清製作與應用
Other Title
Preparation and Application of Polyclonal Antibody on Lettuce mosaic virus
Journal
台灣農業研究
Journal Volume
64
Journal Issue
2
Pages
159-166
Date Issued
2015-06-08
Date
2015-06-08
Author(s)
Abstract
引起嵌紋、皺葉病徵的萵苣樣品接種至紅藜、白藜等病毒指示植物，經過生物分析、寄主範圍測試、病斑特性觀察及血清學等證據顯示，病因為萵苣嵌紋病毒（Lettuce mosaic virus; LMV）。以人工方式接種單斑系病毒於7科27種植物，其中萵苣、菠菜、豌豆及圓葉菸草均產生系統性嵌紋病徵，奎藜表現系統性黃化斑點病徵，紅藜及千日紅則出現局部壞疽斑點病徵，其餘供試植株均未出現病徵。
Lettuce mosaic virus was isolated and identified from infected lettuce plants (Lactuca sativa var. crisp. L.) by using host reactions, electron microscopy, biological and serological assays in Taiwan. Upon artificial inoculation to 27 plant species belonging to 7 families, the virus infected lettuces (Lactuca sativa var. asparagina, L. sativa var. capitata, L. sativa var. crispa, and L. scariola var. sativa), spinach (Spinacea oleracea), pea (Pisum sativum) and tobacco (Nicotiana benthamiana) showed systemically mosaic symptoms. It developed systemically yellow lesions on Chenopodium quinoa, but only local necrotic lesions displayed on C. amaranticolor and G. glodosa. No other tested plants with symptoms were observed.
Subjects
萵苣
萵苣嵌紋病毒
血清
檢測
Lactuca sativa L.
Lettuce mosaic virus
Antiserum
Detection
Publisher
農業試驗所
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
64-2-8.pdf
Size
1.91 MB
Format
Adobe PDF
Checksum
(MD5):cd7f524fb818ebe54746429f4fb23c01