玉米核酸逢機增殖多型性(RAPD)遺傳歧異分析系統之建立

Other Title
Optimization of Random Amplified Polymorphic DNA Analysis System for Maize (Zea mays L.) Genetic Diversity
Journal
中華農業研究
Journal Volume
44
Journal Issue
3
Pages
251-257
Date Issued
1995-09-03
Date
1995-09-03
Author(s)
胡凱康
Shu Chen 
Kae-Kang Hwu
DOI
URI
Abstract
本研究以玉米自交系(Mo17)為材料，以OPERONA-18為核酸引子(primer)，利用快速省時之空氣溫度循環裝置(Air Thermo-Cycler)進行聚合酵素連鎖反應(Polymerase Chain Reaction, PCR)，希望建立玉米多條帶(band)圖譜之核酸逢機增殖多型性(Random Amplified Polymorphic DNA, RAPD)分析系統，以便進行後續之玉米種原遺傳歧異分析。The objective of this study is to optimize Random Amplified Polymorphic DNA (RAPD) fingerprinting analysis system of maize. Various conditions of polymerase chain reaction were tested in a thermo-cycling instrument using maize inbred line Mo17 and a tenbase random primer.
Subjects
玉米
核酸逢機增殖多型性
Zea mays L.
Random Amplified Polymorphic DNA (RAPD)
Publisher
農業試驗所
Type
journal article
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