使用Real-time PCR 檢測引起西瓜蔓枯病菌的方法評估
Other Title
Evaluation of Real-time PCR Assay for Detection of "Didymella bryoniae"
Resource
台灣農業研究 63(2): 151-158
Journal of Taiwan Agricultural Research 63(2): 151-158
Journal
台灣農業研究
Journal Volume
63
Journal Issue
2
Pages
151-158
Date Issued
2014-06-06
Date
2014-06-06
Author(s)
Abstract
本研究根據西瓜蔓枯病菌（Didymella bryoniae）的rDNA設計出專一性的引子對及利用real-time PCR法與TaqMan螢光探針進行D. bryoniae之檢測並與傳統PCR法作比較，結果顯示real-time PCR法較傳統PCR法靈敏度提高約100倍。
In this study, a specific and highly sensitive real-time PCR assay with TaqMan probe was developed as a tool for the diagnosis of gummy stem blight of watermelon by targeting the rDNA genes of ＂Didymella bryoniae.＂ In addition, the real-time PCR assay was compared to a conventional PCR method with their specificity and sensitivity on the detection of ＂D. bryoniae＂ in culture andin planta.
Subjects
西瓜蔓枯病
Didymella bryoniae
Real-time PCR
診斷技術
Gummy stem blight
Didymella bryoniae
Real-time PCR
Detection
Publisher
農業試驗所
Type
journal article
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