MARKERS FOR CYTOPLASMIC MALE STERILITY (CMS) TRAITS IN CHILIPEPPERS (Capsicum annuum L.). I: MULTIPLEX PCR AND VALIDATION
Journal
SABRAO Journal of Breeding and Genetics
Journal Volume
48
Journal Issue
4
Pages
465-473
Date Issued
2016-12
Author(s)
Tzu-Ying Yeh
S. LIN
H. SHIEH
Y. TEOH
S. KUMAR
Abstract
Chili peppers (Capsicum annuumL.) are an important commercial crop valued for their pungent fruits, which are indispensable ingredients in many cuisines around the world. Cytoplasmic male sterility (CMS) is the most commonly used mechanism to produce commercial hybrid seeds of chili pepper. Using a set of 20 pepper (chili and sweet) CMS lines, their 20 maintainer lines and 10 restorer lines, we examined the validity of fivemale sterile cytoplasm (S-cytoplasm) specific (atp6-SCAR607, Ψatp6-2875coxII-SCAR708, orf456,SCAR130/140) and one restoration-of-fertility(Rf)locus specific (CRF-S870) markers. Out of five markers evaluated, co-dominant sequence characterized amplified region (SCAR) marker (SCAR130/140) was found to be most reliable and reproducible for detection of cytoplasm type (S-cytoplasm vs. normal, N-cytoplasm) in peppers. Hence, this CMS marker (SCAR130/140) and Rflocus associated marker (CRF-S870) were used in a multiplex polymerase chain reaction (PCR) protocol to facilitate efficient screening of cytoplasm types in peppers. This multiplex PCR can be used for very efficient and cost effective screening of a large number of pepper lines at the seedling stage in only 15-20 days to determine distribution of cytoplasm types (S vs. N), by passing tedious and time consumingconventional process involving three seasons in developing testcross F1s (in the first season), growing and obtaining F2seeds (in the second season) and finally examining segregation in the F2progenies (in the third season).
Subjects
Chili pepper
germplasm screening
fertility restoration
hybrid seeds
sweet pepper
Publisher
Society for the Advancement of Breeding Research in Asia and Oceania (SABRAO)
Type
journal article