分蔥潛隱病毒親緣分析及應用巢式聚合酶鏈鎖反應作分群偵測

Other Title
Phylogenetic Analysis and Grouping Detection of Species Shallot latent virus by Nested-PCR
Journal
台灣農業研究
Journal Volume
67
Journal Issue
3
Pages
270-282
Date Issued
2018-09-04
Date
2018-09-04
Author(s)
林羿廷
蔡錦慧
鄧汀欽 
Yi-Ting Lin
Chin-Hui Tsai
Ting-Chin Deng 
DOI
URI
Abstract
搜尋美國國家生物技術資訊中心(National Center for Biotechnology Information; NCBI) GenBank登錄之分蔥潛隱病毒種(species Shallot latent virus; SLV)分離株鞘蛋白基因核苷酸序列，進行親緣分析。本研究可將現有SLV分為3個分類群：第一群(G1)、第二群(G2)、第三群(G3)。 The coat protein (CP) gene nucleotide sequences of species Shallot latent virus (SLV) collected from National Center for Biotechnology Information (NCBI)-GenBank database were tested for phylogenetic analysis. All the current SLV isolates tested were clustered into three groups: G1, G2 and G3. In 2015-2016, a total of 10 SLV-infected Allium materials were collected from a survey of SLV occurred in Taiwan by detecting the virus with enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR).
Subjects
分蔥潛隱病毒
蔥科作物
親緣分析
巢式-聚合酶鏈鎖反應
Shallot latent virus
Allium species
Phylogenetic analysis
Nested-polymerase chain reaction
Publisher
農業試驗所
Type
journal article
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