Conservation of Anoectochilus formosanus Hayata by artificial cross-pollination and in vitro culture of seeds
Journal
Botanical Bulletin of Academia Sinica
Journal Volume
43
Journal Issue
2
Pages
123-130
Date Issued
2002-04
Date
2002-04
Author(s)
Abstract
Anoectochilus formosanus is an important ethnomedicinal plant of Taiwan. We have optimized a method for mass propagation of A. formosanus by artificial cross-pollination and asymbiotic germination of seeds. The success of pollination and fruit set was found to be dependent on the developmental stage of male and female gametophytes. Fruit set of hand-pollinated flowers was 86.7%. The seeds from 7-week-old capsules were germinated by culturing on half-strength Murashige and Skoog's (MS) medium supplemented with 0.2% activated charcoal and 8% banana homogenate for four months. Germinated seedlings were cultured in half-strength liquid MS medium containing 2 mg/l N6-benzyladenine (BA) in 125-ml Erlenmeyer flasks for two months. Before ex vitro establishment of seedlings, seedlings with well-developed rhizomes and shoots were cultured on half-strength MS medium with 0.2% activated charcoal, 8% banana homogenate, 2 mg/l BA and 0.5 mg/l a-naphthaleneacetic acid (NAA) for further growth. Around 90% seed-derived plants survived two months after transfer to peat moss:vermiculite potting mixture and incubation in the growth chamber.
Subjects
Anoectochilus formosanus
Conservation
Endangered plant
In vitro propagation
Jewel orchid
Medicinal herb
Orchidaceae
Pollination
Pollinia
Seed germination
Publisher
Institute of Botany, Academia Sinica
Type
journal article