Nutritional and Environmental Conditions for the Growth of Coriolus versicolor, a Wood Decaying and Medical Fungus
Other Title
一種木材腐朽菌及藥用真菌－雲芝，其生長之營養及環境條件之探討
Resource
中華農業研究 39(3): 190-203
Journal of Agricultural Research of China 39(3): 190-203
Journal
中華農業研究
Journal Volume
39
Journal Issue
3
Pages
190-203
Date Issued
1990-09-04
Date
1990-09-04
Author(s)
Ying-Ming Liao
廖英明
Abstract
Coriolus versicolor is a wood decaying fungus, it also has been used as a medicine in chinese tradition to cure many chronic diseases. However, it is also a harmful fungus on the logs in shiitake cultivation. Heavily infection of the logs by C. versicolor easily wiped out the production of shiitake. Three isolates of C. versicolor were used to explore the nutritional requirements and environmental conditions for the mycelial growth and fruiting body formation of C. versicolor. Results of the PDA plate culture indicated that all three isolates grew very well between 28 and 32°C and 12 hours of alternating lights was better than continuous light or darkness. In stationary liquid culture, optimal pH value and C/N ratio were 6.0 and 80. Glucose and mannose were superior to the other carbon sources. Ammonium chloride was best among the nitrogen sources. Urea and sodium nitrite hampered the mycelial growth of C. versicolor. Medium amount in the culture container also affected its mycelial growth. In the 300ml flasks, best growth was found with l20ml of medium or 2.5/1 (v/v).
Among the amino acids used in the mycelial growth tests, DL-asparatic acid was found best and DL-3 : 4-dihydroxy-phenyl-alanine was harmful. In sawdust cultures, best growth was recorded at the moisture content of 54%. Although the cultural filtrate of C. versicolor inhibited the mycelial growth of C. versicolor itself, agar medium containing 1000 or 2000ppm of the cultural filtrate of C. versicolor favored the mycelial growth of shiitake (Lentinus edodes). Formation of fruiting bodies of C. versicolor was observed 4 months after inoculation in the sawdust medium consisting of rice bran, wheat meal and calcium carbonate as it is used in shiitake cultivation.
雲芝（Coriolus versicolor）是一種香菇段木栽培時常見的害菌，但自古以來它也是用作防治慢性病的真菌。一旦受其感染，則該段木幾無收成可言。三個雲芝菌株在PDA plate上培養結果顯示，最適溫度為28─32°C，且在12小時光照，12小時黑暗時，菌絲生長最好。故在探討營養要求方面之液體培養試驗時均在28°C下，靜置培養21天，培養結果顯示Glucose及Mannose為最佳碳源，Ammonium chloride為最佳氮源，但Urea及Sodium nitrite反不利C. versicolor菌絲之生長。在所有供試之氨基酸中，DL-asparatic acid有利雲芝菌絲生長，DL-3：4-dihydroxy-phenyl-alanine則不利。而在pH值為6.0及C/N ratio為80時菌絲生長最好。培養液容量也會影響菌絲生長，在300ml的三角瓶中，若含120ml的培養液，則可得到最多之菌絲乾量。而木屑培養時最適當之含水量為54％。本菌其培養液會影響其自身之生長，但是若以1,000ppm或2,000ppm之量混合在固體培養基中，反有利香菇菌絲之生長。本試驗之三個菌株都可在栽培香菇用之木屑培養基中產生子實體，培養期約四個月。木屑培養基中含有米糠；麥粉及碳酸鈣等添加物。
Subjects
Environmental Condition
Coriolus versicolor
Medical fungus
環境條件
雲芝
藥用真菌
Publisher
農業試驗所
Type
journal article
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