Protein Synthesis in Isolated Mitochondria of Rice (Oryza sativa L.) Seedlings
Resource
Plant Physiology 96(1): 319-323
Journal
Plant Physiology
Journal Volume
96
Journal Issue
1
Pages
319-323
Date Issued
1991-05
Date
1991-05
Author(s)
Hwa Dai
Yih-Shan Lo
Chiung-Yuan Wu
Chia-Lin Tsou
Gon-Shi Hsu
Chyr-Guan Chern
Manfred Ruddat
Kwen-Sheng Chiang
Abstract
For studies of in organello mitochondrial protein synthesis in rice, Oryza sativa L., conventional surface-sterilization procedures were demonstrated to be ineffective. Because of the overwhelmingly efficient [S-35]methionine utilization by contaminating bacteria, even "essentially bacteria-free" rice mitochondria were shown to be unsuitable for the study of in organello protein synthesis. We developed a procedure to obtain a bacteria-free preparation of rice mitochondria. Such mitochondria favored a membrane-dependent ATP-generating system over an external ATP-generating system as the energy supplement for in organello protein synthesis. Two distinct classes of [S-35]methionine-labeled, cycloheximide-insensitive products were detected: an electrophoretically unresolved population and a set of some 22 to 27 discrete polypeptide species, each with a characteristic electrophoretic mobility and relative abundance.
Publisher
American Society of Plant Biology
Type
journal article