Macrophage Mediated Anti-Proliferation Effects of Anthodia camphorata Non-Polysaccharide Based Extracts on Human Hepatoma Cells

Chia-Yu CHANG; Tain-Junn CHENG; Fang-Rong CHANG; Hsien-Yi WANG; Wei-Chih KAN; Shun-Lai LI; Li-Hsueh HUANG; Yu-Chun CHEN; Wan-Chen TSAI; Chia-Hsin HUANG; Chia-Hui CHENG; Guo-Yang LEE; Shiang-Wei SHYUE; Yi-Peng CHEN; Kao-Chang LIN; Jiunn-Jye CHUU

doi:10.1271/bbb.100559

Macrophage Mediated Anti-Proliferation Effects of Anthodia camphorata Non-Polysaccharide Based Extracts on Human Hepatoma Cells

Journal

Bioscience Biotechnology and Biochemistry

Journal Volume

75

Journal Issue

4

Pages

624-632

Date Issued

2011-04

Date

2011-04

Author(s)

Chia-Yu CHANG

Tain-Junn CHENG

Fang-Rong CHANG

Hsien-Yi WANG

Wei-Chih KAN

Shun-Lai LI

Li-Hsueh HUANG

Yu-Chun CHEN

Wan-Chen TSAI

Chia-Hsin HUANG

Chia-Hui CHENG

Guo-Yang LEE

Shiang-Wei SHYUE

Yi-Peng CHEN

Kao-Chang LIN

Jiunn-Jye CHUU

DOI

10.1271/bbb.100559

URI

https://scholars.tari.gov.tw/handle/123456789/14329

https://academic.oup.com/bbb/article/75/4/624/5949912

Abstract

It has been reported that medicinal mushrooms might induce different types of immune responses. Anthodia camphorata (A. camphorata) has attracted much attention for its therapeutic effects in treating hepatoma. We tested this anti-tumor effects using immunomodulation of macrophages and extracts of A. camphorata. We evaluated the anti-proliferation effects of various extracts of A. camphorata from fruiting bodies (AC-FB), mycelium of solid-state cultures (AC-SS), liquid-state cultures (AC-LS) and polyaccharide extracts from liquid-state cultures (AC-PS), and extracts of A. camphorata stimulated RAW 264.7 macrophage cell-conditioned mediums (MC-CMs). We measured cell proliferation and, did migration assays by cell cycle analysis and by observing apoptosis-related proteins (AKT, PARP-1, and NF-κB) and the mRNA expression of cytokines (TNF-α and IL-1β) of macrophages in human hepatoma cell lines. Our results revealed that two of the extracts (AC-FB and AC-SS) had better anti-proliferation effects, implying an immunomodulatory role the macrophages might play. This outcome is consistent with findings that AC-FB and AC-SS increase mRNA expression of TNF-α and the corresponding expression of apoptosis-related proteins on activation of MC-CMs, while A. camphorata polysaccharides induce macrophage-derived anti-tumor activities in human hepatoma cells via IL-1β and Akt activation. These results indicate that anti-tumor effects exerted by modulation of macrophage activation of A. camphorate may be influenced by the other constituents which (contained little or no polysaccharide) of A. camphorata.

Subjects

Antrodia camphorata

polysaccharide

macrophages

immunomodulation

apoptosis

Publisher

Japan Society for Bioscience, Biotechnology, and Agrochemistry

Type

journal article

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