TaqMan real-time PCR for detection and quantitation of squash leaf curl virus in cucurbits

Cheng-Ping Kuan; Hung-Chang Huang; Chia-Che Chang; Yi-Lin Lu

doi:10.1016/j.jviromet.2011.11.024

TaqMan real-time PCR for detection and quantitation of squash leaf curl virus in cucurbits

Journal

Journal of Virological Methods

Journal Volume

179

Journal Issue

2

Pages

367-372

Date Issued

2012-02

Date

2012-02

Author(s)

Cheng-Ping Kuan

Hung-Chang Huang

Chia-Che Chang

Yi-Lin Lu

DOI

10.1016/j.jviromet.2011.11.024

URI

https://scholars.tari.gov.tw/handle/123456789/14338

https://www.sciencedirect.com/science/article/pii/S0166093411004617?via%3Dihub

Abstract

A real-time PCR assay based on the TaqMan chemistry was developed for reliable detection and quantitation of the squash leaf curl virus (SLCV) in melon and squash plants. This method was highly specific to SLCV and it was about one thousand times more sensitive than the conventional PCR method. The protocol of the real-time PCR established in this study enabled detection of as little as 102 copies of SLCV DNA with CP gene as the target. This TaqMan real-time PCR assay for detection and quantitation of SLCV would be a useful tool for application in quarantine and certification of SLCV in cucurbits as well as in the research of disease resistance and epidemiology.

Subjects

Squash leaf curl virus

Squash

Cucurbita pepo

Melon

Cucumis melo

Real-time PCR

Publisher

Elsevier

Type

journal article

File(s)

Name

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Size

22 KB

Format

HTML

Checksum

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