偵測荔枝酸腐病菌之選擇性培養基
Other Title
A Selective Medium for the Isolation of Geotrichum Candidum and Geotrichum Ludwigii from Litchi and Soil
Journal
植物病理學會刊
Journal Volume
8
Journal Issue
1
Pages
9-14
Date Issued
1999-03
Author(s)
Abstract
Geotrichum candidum 及 Geotrichum ludwigii 是引起荔枝酸腐病之病原菌，在 選藥劑防治病時發現腐絕（thiabendazole）及依普同（iprodione）兩種藥劑，在防治果樹 病害推荐濃度（分別為800∼1000 ppm 及160 ppm）下對本病原菌並無抑制作用，而此兩種 藥劑對於多種真菌皆有殺菌效果。以此兩種藥劑合成選擇性培養基，可有效偵測植殘體及土 壤中病原菌之存在。將病原菌拌入滅菌後及田間之自然土壤，再以選擇性培養基偵測，其回 收率G. candidum 及 G. ludwigii 分別為98.7% 及98.3%。 選擇性培養基之配方以馬鈴薯 葡萄糖瓊脂培養基（potato dextrose agar）為主、添加乳酸（75% lactic acid）1250 ppm 、腐絕8000 ppm 、依普同240 ppm 及玻瑰紅（rose bengal）50 ppm。
A selective medium was developed for isolation of Geotrichum candidum and Geotrichum ludwigii, the cause of sour rot of litchi, from naturally infested soil and fruits. The recovery rates of G. candidum and G. ludwigii from the artificially infested soil were 98.7% and 98.3%, respectively. The medium consists of potato dextrose agar (200 g potato, 20 g dextrose and 15 g of Bacto agar in 1000 ml distilled water), 1250 ppm of lactic acid, 8000 ppm of thiabendazole, 240 ppm of iprodione and 50 ppm of rose bengal.
Subjects
荔枝酸腐病菌
選擇性培養基
Sour rot of litchi
Geotrichum candidum
Geotrichum ludwigii
selective medium
Publisher
中華植物病理學會
Type
journal article