Identification of Anoectochilus formosanus and Anoectochilus koshunensis Species with RAPD Markers

Kur-Ta Cheng; Li-Chung Fu; Chang-Sheng Wang; Feng-Lin Hsu; Hsin-Sheng Tsay

doi:10.1055/s-2006-957364

Identification of Anoectochilus formosanus and Anoectochilus koshunensis Species with RAPD Markers

Journal

Planta Medica

Journal Volume

64

Journal Issue

1

Pages

46-49

Date Issued

1998-02

Date

1998-02

Author(s)

Kur-Ta Cheng

Li-Chung Fu

Chang-Sheng Wang

Feng-Lin Hsu

Hsin-Sheng Tsay

DOI

10.1055/s-2006-957364

URI

https://scholars.tari.gov.tw/handle/123456789/1771

Abstract

RAPD (random amplified polymorphic DNA) markers were developed to distinguish Anoectochilus formosanus from Anoectochilus koshunensis and their putative hybrids. Morphological differentiation of these two species beyond the flowering period is difficult. RAPD markers provide a rapid and easy tool for identification of the two Anoectochilus species. In the study, forty arbitrary decamer primers were screened, and nineteen species-specific RAPD markers generated from polymerase chain reactions (PCR) with eight random primers were obtained. Nine were specific to A. formosanus and ten to A. koshunensis. Two primers, OPC-08 and OPL-07, produced two markers, one specific to A. formosanus and the other specific to A. koshunensis, which simultaneously appeared in the hybrids pattern. The RAPD markers can be applied both to identification of A. formosanus and A. koshunensis species and to assessment of the extent fo hybridization in hybrids between them. This information facilitates the breeding program process.

Subjects

Anoectochilus

Orchidaceae

RAPD

hybrid

identification

Publisher

Georg Thieme Verlag

Type

journal article

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