晚香玉花藥培養及其癒合組織再生株之變異
Other Title
Anther culture and somaclonal varivation of tuberose (Polianthes tuberosa L.)
Journal
中華農業研究
Journal Volume
38
Journal Issue
3
Pages
346-352
Date Issued
1989-09-07
Date
1989-09-07
Author(s)
紀海珊
蔡新聲
H.S. Gi
H.S. Tsay
Abstract
晚香玉單瓣栽培種（Polianthes tuberosa L . cv “Single”）單核期的花藥，接種於含有l ppm 2，4-D、3％蔗糖及0.8％洋菜之1/2 MS基本鹽類（Na-Fe-EDTA全量）培養基中，可獲得具有胚發生能力之癒合組織（embryogenic callus）。將此癒合組織移植於植物生長調節劑為0.2 ppm NAA及2 ppm kinetin 之1/2 MS 基本鹽類培養基，可獲得擬胚體及再生植株。所獲得35株之再生值株，據調查已開花8株之園藝性狀，顯示有花序增長、花蕾數增加、花徑變大、花瓣數變少、外花被呈粉紅色及葉片大小的差異，此變異或可供為改良晚香玉單瓣種花序短、花蕾數少及切花品質低等缺點之選拔材料。
Anthers of tuberose variety with single-petal flowers was used in this experiment for callus induction and plant regeneration. Anthers with microspores at uninucleate stage were cultured on medium of ½ strength MS basic salts (with full strength of Na-Fe-EDTA) supplemented with lppm 2, 4-D, 3% sucrose and 0.8% phytagar. The produced embryogenic callus was able to differentiate into embryoids and plantlets after transfering to medium containing 0.2ppm NAA and 2ppm kinetin. A total of 35 plant-lets were produced by the procedures described above. Among them, 8 plants grew to bloom showing significant variaton in various floral traits. These included inflorescence length, number of flower buds, flower size and color. It is suggested that the plants produced from tissue culture can be used as a source for varietal improvement.
Publisher
農業試驗所
Type
journal article
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