誘引劑對高氏柴胡懸浮細胞生長與柴胡皂苷含量累積之影響
Other Title
Influence of Elicitors on Cell Proliferation and Saikosaponin Production of Bupleurum kaoi in Suspension Culture
Resource
台灣農業研究 61(2): 112-123
Journal of Taiwan Agricultural Research 61(2): 112-123
Journal
台灣農業研究
Journal Volume
61
Journal Issue
2
Pages
112-123
Date Issued
2012-06-04
Date
2012-06-04
Author(s)
陳威臣
夏奇鈮
葉茂生
曹進義
蔡新聲
Uei-Chern Chen
Chi-Ni Hsia
Mau-Shing Yeh
Chin-Yi Tsao
Hsin-Sheng Tsay
Abstract
高氏柴胡（Bupleurum kaoi Liu, Chao et Chuang）懸浮細胞於含有0.2 mg/L二氯苯氧基乙酸（2,4-dichlorophenoxyacetic acid, 2,4-D）之1/2 MS液態培養基中於低光照下以100 rpm轉速培養，細胞之生長在接種後0-2週為緩慢生長期，在2-4週呈現快速生長期，在培養4週後進入生長靜止期。
Bupleurum kaoi, a native medicinal plant in Taiwan which contains saikosaponins higher than other commercial Bupleurum species, was chosen for producing saikosaponins in vitro. Cell suspension cultures of B. kaoi were cultured in a half-strength MS liquid medium containing 0.2 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and used for studying effect of speed of shaker, size of flasks and inoculum density on cell proliferation as well as studying effect of elicitors, yeast extract (YE), abscisic acid (ABA), and jasmonic acid (JA), on saikosaponin production.
Subjects
高氏柴胡
藥用植物
懸浮細胞培養
柴胡皂苷
誘引劑
Bupleurum kaoi
Medicinal plant
Cell suspension culture
Saikosaponin
Elicitor
Publisher
農業試驗所
Type
journal article
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