鹽類濃度、蔗糖、植物生長素及培容器瓶口覆蓋物對高氏柴胡組培苗發根與馴化之影響
Other Title
Influence of Salt Strength, Sucrose, Auxins and Container Closure on Root Formation and Acclimation of In Vitro Bupleurum kaoi Plantlets
Resource
中華農業研究 53(4): 249-260
Journal of Agricultural Research of China 53(4): 249-260
Journal
中華農業研究
Journal Volume
53
Journal Issue
4
Pages
249-260
Date Issued
2004-12-04
Date
2004-12-04
Author(s)
陳威臣
夏奇鈮
葉茂生
蔡新聲
Uei-Chern Chen
Chi-Ni Hsia
Mau-Shing Yeh
Hsin-Sheng Tsay
Abstract
本研究探討培養基組成中之MS (Murashige & Skoog)鹽類濃度、蔗糖濃度與植物生長素，對高氏柴胡(Bupleurum kaoi Liu, Chao et Chuang)組培苗發根之影響，並藉由瓶口覆蓋物處理降低瓶內溼度，以提高瓶苗移植存活率之方法，建立高氏柴胡組織培養之大量繁殖種苗體系。
An efficient protocol for producing high quality rooted plantlets which are easy to acclimatize for field growth was established for micropropagation of Bupleurum kaoi Liu, Chao et Chuang. In this study, the medium containing half- and quarter- strength of MS basal salts (1/2MS and 1/4MS) and 3% (w/v) sucrose was found suitable for shoot survival and root formation of in vitro B. kaoi plantlets.
Subjects
高氏柴胡
藥用植物
發根
馴化
培養容器瓶口覆蓋物
Bupleurum kaoi
Medicinal plant
Rooting
Acclimation
Container closure
Publisher
農業試驗所
Type
journal article
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