Determination of the components in a Chinese prescription, Yu-Ping-Feng San, by RAPD analysis
Journal
Planta Medica
Journal Volume
64
Journal Issue
6
Pages
563-565
Date Issued
1998-08
Date
1998-08
Author(s)
Kur-Ta Cheng
Hsin-Sheng Tsay
Chieh-Fu Chen
Tsue-Wei Chou
Abstract
In this study, the RAPD (random amplified polymorphic DNA) technique was employed for the first time to determine the components in a Chinese herbal prescription. Forty decamer oligonucleotide primers were screened in the RAPD analysis to identify three Chinese medicines, the dried root of Astragalus membranaceus (Fisch.) Bge., the dried root of Ledebouriello seseloides Wolff, and the dried rhizome of Atractylodes macrocephala Koidz, in a Chinese prescription. Only primer OPP-10 simultaneously generated three distinct markers were each specific to one component. The marker with 200 bp is specific to Astragalus membranaceus; the 440 bp marker is specific to Atractylodes macracephala; and the remaining marker with 500 bp was present in Ledebouriello seseloides. The presence of the three herbal medicines in the mixed sample, the Chinese prescription, was determined when the primer OPP-10 RAPD reaction was performed. The technique was proved to contribute to the identification of components in the Chinese medicinal preparations.
Subjects
RAPD
identification
Chinese herbal prescription
Astragalus membranaceus
Ledebouriella seseloides
Atractylodes macrocephala
Publisher
Georg Thieme Verlag
Type
journal article