植物生長調節劑及培養基型態對湖北貝母鱗片培養衍生芽球相似體及小鱗片增重之影響
Other Title
Effects of Plant Growth Regulators and Status of the Medium on Induction and Proliferation of Protocorm-Like-Bodies and Bulblets from Bulbscale Culture of Fritillaria Hupehensis Hsiao et K. C. Hsia
Journal
中華農學會報
Journal Volume
2
Journal Issue
5
Pages
414-422
Date Issued
2001-10
Date
2001-10
Author(s)
楊淑如
戴宗德
陳忠川
蔡新聲
Shu Ru Yang
Chung-Der Tai
Chung-Chuan Chen
Hsin-Sheng Tsay
Abstract
湖北貝母在物分類學上屬百合科(Liliaceae)、貝母屬(Fntillaria)之多年生草本物，多以地下鱗莖(bulbscale)入藥，為重要且廣泛使用之中藥，歷年來均以鱗莖繁殖，但因鱗莖繁植倍率低，子繁殖又需4~5年之生長週期，因此造成藥品嚴重缺乏，不足以滿足醫療之需。本研究以組織培養方式繁殖北貝母，除了希望能達到大量繁殖之目的外，也期望對湖北貝母由鱗莖所誘導之芽球相似體(protocorm-like-body，PLB)及小鱗片(bulblet)重量之增加有所助益以有益於藥用資源之開發利用。結果顯示，將湖北貝母鱗莖培養在含有0.5 mg/l kinetin之基本MS固體培養基中，可誘導形成含PLB及小鱗片(bulblet)之團塊(PLB mass)及合組織；而添加適量之2,4-D或NAA於含有0.5 mg/l kinetin之固體培養基中，對PLB mass及合組織之形成有促進之效果，NAA之效果又優於2，4-D，其中尤以4 mg/l NAA之效果最佳。基本MS培養基添加植物生長調節劑對湖北貝母芽球相似及小鱗片增重之影響，則以0.5~1.0 mg/l NAA配合0.5 mg/l BA效果最好-進一步再以添加0.5 mg/l BA及0.5 mg/l NAA的基本MS培養基測試固體或液
體培養基對湖北貝母芽球相似體及小鱗片增重之影響，結果發現液體培養之效果優於固體：在容量為125 ml之三角瓶加入5、10或15 ml液體培養基之三種處理中，在60 rpm速率振盪下之增重效果以5 ml之處理最佳，芽球相似體及小片經培養30天後的増重倍率分別為19.6倍與23.7倍。
Fnrillaia hupehensis (Liliaceae), commonly known as Bel-mu in Chinese, is one of the important traditional Chinese medicinal plant. It is propagated by bulbscales. As the amount of bulbscales collected from naturally-grown and traditionally-cultured plants is far from enough for medicinal demand, we have optimized a method of rapid mass propagation of Bei-mu Using bulbscales. Callus and plotocom-like-bodies (PLB) were induced by culturing the bulbscales on Murashige and Skoog (MS) medium supplemented with 0.5 mg/1 6-furfunylamino purine (kinetin). PLB mass when cultured on MS medium with either 0-8 mg/1 2,4-dichlorcphenaxyacetic acid (2,4-D) or (-naphthaleneacetic acid (NAA) in combination with mg/l kinetin proliferated producing bulblets and callus. NAA was found to be superior to 2,4-D both PLB mass proliferation and callus formation. PLE mass was cultured on MS medium supplemented with 0.1-4.0 mg/l NAA in combination with 0.5 mg/1 benzylaminpure (EA) for further proliferation and increase in the fresh weight of PLE was recorded after 60 days of culture in light. The PLB mass and bulblets were also cultured in liquid MS medium supplemented with 3% sucrose, 0.5 mg/l BA and 0.5 mg/l NAA in 125-ml Erlenmeyer flasks with 5-15 ml medium. The amount of liquid medium and state of the medium (liquid vs. solid) were found to influence the proliferation of PLB mass and bulblets.
Subjects
貝母
小鱗片
湖北貝母
中草藥
大量繁殖
癒合組織
芽球相似體
Bei-rnu
bulbscale
Fritillaria hupehensis
medicinal herb
micrcpropagation
callus
protocorm-like-body
Publisher
中華農學會
Type
journal article