Mycophasma-like Organisms Assoeiated With Witches’ Broom of Wild Balsam Pear in Taiwan
Other Title
臺灣野苦瓜簇葉病之擬菌質體病原
Journal
中華農業研究
Journal Volume
36
Journal Issue
2
Pages
216-221
Date Issued
1987-06-09
Date
1987-06-09
Author(s)
I-Lang Yang
Moh-Jih Chen
Shuang-Ming Wang
楊一郎
陳脉紀
王雙明
Abstract
The witches’ broom disease on wild balsam pear was newly found in an isolated areas near Shui-li, Nan-tou county in the central Taiwan in 1984. The diseased plants showed typical witches’ broom symptoms comprising bushy little leaves, green flowers and abnormal conical shoots. Numerous pleomorphic bodies, characteristic of mycoplasma-like organisms (MLO), were observed in veinal sieve elements of the diseased leaves under electron microscopy. They were spherical, ovoid or ellipsoidal about 110 to 740 nm in diameter. No such MLO bodies were found in the healthy plant. The stem and leaf sections were observed directly under a reflecting fluorescence microscope and also examined under light microscope after treatment with Dienes’ stain. The specific yellow fluorescence and blue staining reaction were noticed in affected phioem cells, but no such reactions were detected in those of healthy. The results revealed the presence of MLOs in the sieve elements were in accord with what observed under electron microscopy. We suggest an MLO etiology for the witches’broom of wild balsam pear.
臺灣之野苦瓜簇葉病（Wild balsam pear witches ' broom)，係第一作者於1984年，在南投水里地區發現之新病害。病株叢生小葉、異常分蘗、綠化花，顯現典型簇葉病徵。田間採集之病株葉脈組織超薄切片之電子顯微鏡觀察結果，篩管細胞內有多數多形性擬菌質體（mycoplasma-like organisms）存在，呈球形、惰圓形及圓形等大小不同之各種形態，直徑約110-740nm。健全株則沒有擬菌質體。病株莖葉徒手切片，經Dienes' stain染色，並以光學顯微鏡觀察，結果在罹病篩管細胞內，顯現專一性藍色之Dienes' stain染色反應，健株者則無此反應。另將病組織徒手切片，直接以反射型螢光顯微鏡觀察結果，在篩管細胞，呈現顯著特殊螢光反應，這些特殊螢光反應局限於篩管組織，健全株之篩管組織則無此反應。上述專一性反應表示其擬菌質體之存在，其存在位置及數量與電子顯微鏡觀察結果相似。以上結果顯示擬菌質體似為野苦瓜簇葉病之病原。
Publisher
農業試驗所
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
journal_arc_36-2-9.pdf
Size
1.18 MB
Format
Adobe PDF
Checksum
(MD5):01e952949e65deb24c3c0452ee6d8f29