Dodder Transmission and Microscopic Observations of Red-bird Cactus Witches’ Broom Disease
Other Title
青龍簇葉病之菟絲媒介傳染與顯微鏡觀察
Journal
中華農業研究
Journal Volume
38
Journal Issue
4
Pages
463-467
Date Issued
1989-12-09
Date
1989-12-09
Author(s)
I-Lang Yang
楊一郎
Abstract
The red-bird cactus witches’ broom (RbWB) was transmitted from infected red-bird cactus to periwinkle by the dodder Cuscuta chinensis Lam. Free hand sections of RbWB infected periwinkle and red-bird cactus showed specific blue staining of Phloem cells with Dienes’ stain under a light microscope, and specific bright fluorescence of phloem tissues under a reflecting fluorescence microscope. Similar sections from healthy plants gave no reaction, which indicated the presence or infections of mycoplasma-like organisms in the phloem tissues of infected plants. On periwinkle, red-bird cactus witches’ broom induced a mild degree of bushy growth.
青龍簇葉病(Red-bird cactus witches’broom)可經菟絲(Cuscuta chinensis Lam.)媒介傳染而使長春草(Vinca rosea L.)感染罹病顯現葉化花之輕型叢生病徵。感染青龍簇葉病之長春草及青龍之病株切片,分別以經Dienes’stain 處理後光學顯微鏡觀察及直接以反射型螢光顯微鏡觀察之結果,在其病組織篩管細胞分別顯現專一性藍色反應及特殊黃色螢光反應,對照健株則無此項反應,此項反應表示病株篩管組織中擬菌質病原之存在。
Publisher
農業試驗所
Type
journal article
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