Leaf, stem and crown galls on perennial asters caused by Agrobacterium tumefaciens in Taiwan

Fure-Chyi Chen; Shiow-Huey Hseu; Shio-Tau Hung; Ming-Chao Chen; Chien-Yih Lin

Leaf, stem and crown galls on perennial asters caused by Agrobacterium tumefaciens in Taiwan

Journal

Botanical Bulletin of Academia Sinica

Journal Volume

40

Journal Issue

3

Pages

237-242

Date Issued

1999-07

Date

1999-07

Author(s)

Fure-Chyi Chen

Shiow-Huey Hseu

Shio-Tau Hung

Ming-Chao Chen

Chien-Yih Lin

URI

https://scholars.tari.gov.tw/handle/123456789/4498

https://ejournal.sinica.edu.tw/bbas/toc/issue-1999-3.html

Abstract

Leaf, stem, and crown galls induced by Agrobacterium tumefaciens were observed on field grown perennial asters (Aster spp.). Plants of purple flowers were more susceptible to infection than white flowers. The occurrence ranged from 25% in white flowered and 90% in purple flowered plants. Galls also occurred on leaves wounded by insect bites or mechanical shearing. Agrobacterium tumefaciens was isolated from crown and leaf galls with a selective medium NASA. The bacterial isolate was identified as A. tumefaciens using the Biolog GN system. Inoculation of selected A. tumefaciens strains on Kalanchoe pinnata leaves resulted in gall formation 8~10 days afterward. Several other A. tumefaciens strains from different gall samples also caused gall formation 6~8 days after inoculating on the stems of tobacco, and tomato seedlings. Re-inoculating virulent strains by scissors onto healthy aster leaves also induced galls 10 to 12 days after cut-inoculation. Biochemical tests of most Agrobacterium strains from aster galls showed that they belong to biovar 1.

Subjects

Agrobacterium tumefaciens

Aster sp.

Biovar 1

Crown gall

Selective medium

Publisher

Institute of Botany, Academia Sinica

Type

journal article