台灣地區矮化線蟲新記錄種Tylenchorhynchus zeae (Nematoda: Belonolaimidae)之鑑定
Other Title
Identification of a new recorded stunt nematode Tylenchorhynchus zeae (Nematoda: Belonolaimidae) in Taiwan
Journal
植物病理學會刊
Journal Volume
16
Journal Issue
2
Pages
79-86
Date Issued
2007-06
Date
2007-06
Abstract
自2006年8月起，從台灣4個鄉鎮地區的玉米、甘藍及白花椰菜等作物的根圈土壤中共分離鑑定6群Tylenchorhynchus zeae Sethi & Swarup, 1968，此一矮化線蟲為台灣地區的新記錄種。各群矮化線蟲之主要鑑定依據為外觀形態和形態測量值(morphometrics)，並輔以核糖體DNA(ribosomal DNA, rDNA)片段（包括完整5.8S基因，內轉錄區間ITS-1和ITS-2及部分18S和28S基因）鹼基序列的相似度比對分析。本研究提供較副模標本更多的形態測量值（包括b'、m、o、EP、P、tail及ABW等共7項）和SEM外觀形態資料，並首次在GenBank中登錄T. zeae的rDNA片段代表性序列，大小為1151bP，其中ITS-1、ITS-2和5.8S的序列大小依序分別為459bp、225bp及161bp。
Six populations of Tylenchorhynchus zeae Sethi & Swarup, 1968 derived from soil samples collected from the rhizosphere of corn, cabbage, and cauliflower, is a new record species from Taiwan. Based on the external morphology, morphometrics and the sequences of ribosomal DNA (including complete 5.8S gene, internal transcribed spacer ITS-1, ITS-2, and partial 18S and 28S gene), each nematode population was confirmed to the species level. In this study, additional morphometric data and SEM figures for this stunt nematode were also included. The representative sequences of rDNA fragment of T .zeae, with a total length of 1051 bp, which including ITS-1 (459 bp), ITS-2 (225 bp) and 5.8S gene (161 bp), were the first to be submitted to the GenBank.
Subjects
矮化線蟲
鑑定
形態測量值
核糖體DNA
內轉錄區間
Tylenchorhynchus zeae
stunt nematode
identification, ribosomal DNA
internal transcribed spacers
Tylenchorhynchus zeae
Publisher
中華民國植物病理學會
Type
journal article
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