蝴蝶蘭瓶苗棉塞密度對實生苗生長之影響
Other Title
Density of Cotton Plug Density on the Growth of Phalaenopsis Seedlings
Journal
臺灣園藝
Journal Volume
52
Journal Issue
2
Pages
187-198
Date Issued
2006-06
Date
2006-03
Author(s)
Abstract
本研究收集4 個國內蝴蝶蘭瓶苗生產單位培養瓶之橡膠塞，分析其棉花密度。結果棉花塞密度分佈最低為 154 mg/cm 3， 最高為 573 mg/cm 3。 其中分佈於 201-400 mg/cm 3 者佔 72%。另以棉花塞密度為 19-573 mg/cm 3 之培養瓶，培養蝴蝶蘭種苗。器內 CO2 濃度之日韻律變化及氣孔行為，顯示實生苗於培養 3 個月後可進行C 3 型代謝。其根之乾/鮮重比值，隨棉花密度提高而降低；莖葉之乾/鮮重比值，則以76 mg/cm 3 棉花密度者最高。在 280 mg/cm 3 棉花塞密度下培養之植株，葉綠體呈長紡錘形，類囊體膜較429 mg/cm 3 棉花塞密度下者排列緊密，澱粉粒及質體小球數目則較少且較小。植株葉背之氣孔密度雖然不因棉花密度而改變，但培養在棉花密度較低的蝴蝶蘭，氣孔在明暗期有開閉度變化。依試驗結果，建議培養蝴蝶蘭瓶苗之棉花塞密度為76 mg/cm 3 。
The cotton plug density of rubber stoppers, collected from 4 local phalaenopsis tissue culture nurseries, ranged from 154 to 573 mg/cm 3 , and 72 % of them were 201 400 mg/cm 3 . The diurnal change of CO 2 concentration and stomata behavior showed that seedlings performed C3 metabolism after 3 months of in vitro culture, regardless of cotton plug density between 19 and 573 mg/cm 3 . The root fresh/dry weight ratio decreased as cotton density increased, and plantlets cultured with 76 mg/cm 3 cotton plugs showed the highest shoot fresh/dry weight ratio. Plants cultured in flask with 280 mg/cm 3 cotton plug density had long spindleshaped chloroplasts, which had fewer and smaller starch grains and plastoglobulins than that cultured in flask with 429 mg/cm 3 cotton plug density. Stomata density did not change as cotton plug density increased from 280 to 429 mg/cm 3 . However, stomata opening/closure was more pronounced in flasks with 280 than with 429 mg/cm 3 cotton plug density. Therefore, the cotton plug density with 76 mg/cm 3 was recommended for phalaenopsis culture.
Subjects
蘭苗
通氣性
二氧化碳
葉綠體
orchid seedling
ventilation
CO 2
chloroplast
Publisher
臺灣園藝學會
Type
journal article