水稻不同次位分蘗、穗位及枝梗間花葯培養癒傷組織之形成

Abstract

以臺農67號與臺南5號兩水稻栽培品種爲材料，利用兩種不同取樣方式比較不同次位分蘖，不同穗位及不同梗梗間花莉癒傷組織的形成能力。第一種取樣方式是在同一株水稻中，從主桿到第三次分蘖，隨著其發育而依序取樣，以此種取樣方式，主桿與分茶間之花滿培養癒傷組織形成能力極爲一致；第二種取樣方式是每一株水稻只取一特定次位分藥，其餘分蘖則轉較為是保韶在植株上，此種取樣的結果，主桿與一、二次分aynd花莉癒傷組織的形成能力仍大致相同，三次分蘖則較爲低下。第二種取樣方式同時顯示對於開花末期接種的花藥癒傷組織形成能力有不利的影響。The objective of this study was to compare the callus-forming ability of anthers originated from rice tillers of different orders; pikelets at different position and branches. Two methods of sampling panicles for anthers culture were employed. The first method was to collect panicles at the appropriate developmental stage from rice tillers of different orders within the same plant. The second method was to harvest panicles from tillers of a definite order from individual plant, vegetative organs were remained in both sampling methods.