https://scholars.tari.gov.tw/handle/123456789/12363| Title: | 甜瓜逢機增幅多型性核酸(RAPD)標誌分析及其遺傳特性之研究 | Other Titles: | A Study of Random Amplified Polymorphic DNA and Its Genetic Characteristics in Cucumis melo | Authors: | 劉邦基 張有明 許華欣 鄭櫻慧 張慧如 蕭吉雄 Pan-Chi Liou You-Ming Chang Wha-Shin Hsu Ying-Huey Cheng Hwei-Ru Chang Chi-Hsiung Hsiao |
Keywords: | 甜瓜;聚合酶連鎖反應;逢機增幅多型性核酸標誌;遺傳分析;Cucumis melo L.;Polymerase chain reaction (PCR);Random amplified polymorphic DNA (RAPD);Genetic analysis | Issue Date: | 5-Dec-1998 | Publisher: | 農業試驗所 | Journal Volume: | 47 | Journal Issue: | 4 | Start page/Pages: | 377-387 | Source: | 中華農業研究 | Abstract: | 本研究以一粗包含三個世代之甜瓜族群為材料，進行甜瓜的逢機增幅多型性核酸(random amplified polymophic DNA, RAPD)分析，並探討其特性。試驗中證明甜瓜範本核酸在PCR反應前先以限制酶酶處理會改變RAPD條帶數目，使分析圓譜發生變化，增加多型性之顯示機率，因此可提高分析效率。 In this paper we reported some genetic characteristics in Cucumis melo based on the analysis of random amplified polymorphic DNA (RAPD) markers using a 3-generation population. It has been found in this study that the variation of RAPD banding patterns will occur if restriction enzyme was used for template DNA digestion before PCR. |
URI: | https://scholars.tari.gov.tw/handle/123456789/12363 | ISSN: | 0376-477X | DOI: | 10.29951/JARC.199712.0013 |
| Appears in Collections: | 1.台灣農業研究(1950～迄今) |
| File | Size | Format | |
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| 47-4-5.pdf | 3.4 MB | Adobe PDF | View/Open |
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