栽培稻與野生稻之雜交第一代胚培養

Abstract

在許多秈、稉稻與野生稻之雜交試驗中，稉稻臺農67號(TNG67)×野生稻（O. officinalis 之W37品系）雜交組合之雜種胚培養結果最佳，授粉後7~9天的雜交胚，癒合組織形成率及植株再生率最高；將雜交胚培養於N6無機鹽類及MS有機鹽類培養基，添加4mg/1 NAA及1mg/1 kinetin，可直接誘導雜交胚產生F1植株，而固體、液體培養對大量繁殖F1植株的效果類似，但植株存活率以固體培養較佳。The frequency of callus formation and the subsequent plant regeneration from 7 to 9 day old embryo cultured in vitro, the cross between cultivar Tainung 67 of O. sativa and strain W37 of O. officanalis, was superior to the other seven crosses within the two distantly related species. The medium containing N6 inorganic salts, MS organic substance, supplemented with 4 mg/1 NAA and 1mg/1 kinetin facilitated the direct production of plantlets from the cultured hybrid embryos. Culture of the hybrid plantlets on solid or liquid medium showed no significant difference in their multiplication rate. However, the survival rate was higher on the solid than in the liquid medium.