免疫酵素分析測定臺灣馬鈴薯病毒之發生與優良品系之抗Y病毒反應

Abstract

利用酵素結合抗體免疫分析(ELISA)檢測馬鈴薯病毒A(PVA)、S(PVS)、X(PVX)、及Y之嵌紋型系統(PVY-M)和壞疽型系統(PVY-N)在臺灣馬鈴薯產區之發生情形，結果顯示病毒發生並無地區性差異，而在於種薯的來源和品種（系）的不同。本所選育的優良品系No.143、No.770、385095-15、385095-54等皆普遍帶有PVS和（或）PVX，而較少PVY的發生，品系中No.143的PVY-M檢出率尤其偏低；但栽培品種卡地娜和克尼伯則少PVS或PVX的感染，而對PVY有較高的再感染率。Occurrence of potato viruses A (PVA), S (PVS), X (PVX), Y mosaic strain (PVY-M) and Y necrotic strain (PVY-N) in potato growing areas of Taiwan were investigated by ELISA. The frequency of virus detected from sampled locations showed no regional difference, but souces of seed-tubers and varieties cultivated made difference. All promising potato clones of No.143, No.770, 3865095-15, and 3865095-54 were found to be widely contaminated by PVS and/or PVX but much less infected by PVY. Particularly clone No. 143 was rarely infected by PVY-M.