|Title:||豇豆蚜媒嵌紋病毒及其對長豇豆產量及品質之影響||Other Titles:||Cowpea Aphid-borne Mosaic Virus and Its Effect on the Yield and Quality of Asparagus Bean||Authors:||張清安
|Issue Date:||9-Sep-1983||Publisher:||農業試驗所||Journal Volume:||32||Journal Issue:||3||Start page/Pages:||270-278||Source:||中華農業研究||Abstract:||
一種引起長豇豆葉片嚴重嵌紋病徵的毒素病廣泛發現於本省各主要栽培區。罹病植株生長不良，枝葉稀疏，開花及結莢率顯著降低，豆莢較正常株者略短，種子數亦較少。試驗證實此病乃由一種屬於PVY 群之長絲狀病毒感染所致。病毒粒子長度約750nm 。可經由機械接種及桃蚜（Myzus persicae Sulze ）以非永續型方式傳播。病株所生種子帶毒率約為4.7%。病毒粗汁液之耐熱性為55-60℃，耐稀釋性為10-4-10-5 ，於20℃ 下感染力可保持7-8 天。此病毒可感染豌豆等大多數之荳科植物，對茄科的煙草（Nicotiana tabacum）， N. debnyii 及N . sylvestris 可於接種葉造成黃化斑點，對N . rustica及N . bonsamiana 則造成系統性斑紋病徵，莧科之千日紅（Gomphrena globosa ）可於接種葉產生黃化斑點，藜科的Chenopodium amaranticolor , Ch. quinoa 及Ch . mulare 均可做為本病毒之檢定用局部病斑寄主。上述特性與文獻中所記載的豇豆蚜媒嵌紋病毒（Cowpea aphid - borne mosaic virus ，簡稱CAMV）極為類似，且應用SDS 免疫擴散反應與美國之CAMV 抗原比較結果，證實二者血清學特性完全相同（serologically identical），故確定二者乃相同之病毒。網室栽培之情況下，本省長豇豆之主要品種一青皮黑仁紫尖感染CAMV 後每株之豆莢數、種子數及二者之乾重均明顯下降，與不接種對照株間達極顯著之差異（P=0.01），但病株每一豆莢之長度、乾重、所含種子數及種子平均重量則與健株無顯著差異。
A virus causing severe mosaic on asparagus bean (Vigna sesquipedalis Fruwirth) was found widespread in several major production areas in Taiwan. The virus was transmitted mechanically to 9 species in Leguminosae, including pea, mungbean and soybean, but failed to infect peanut and broadbean. In Solanaceae, Nicotiana tabacum cv. Vam-Hicks, N. debnyii and N. sylvestris were infected with the production of local faint chlorotic spots whereas on N. bensamiana and N. rustica systemic mottles were observed. Local lesions were formed on several other plant species including Chenopodium amaranticolor, C. quinoa, C. mulare and Gomphrena globosa. The virus was seed-borned and readily transmitted by green peach aphid (Myzus persicae Sulze ) in a nonpersistent manner. Some physical properties of the virus in crude sap were determined: thermal inactivation point, 55-60°C; dilution end point, 10-4-10-5 ; and aging in vitro, 7-8 days under 20°C. Electron microscopy of leaf-dip preparations revealed that the virus was flexuous rod-shaped with a length of 750 nm. All these properties are very similar to those of cowpea aphid-borne mosaic virus reported in many other countries. Compara tive SDS-immunodiffusion tests also proved that the virus was serologically identical to CAMV from Dr. Gonsalves of Cornell Univ., therefore, we concluded that the virus of asparagus bean mosaic in Taiwan is cowpea aphid-borne mosaic virus. In the screen- house experiments, the virus caused significant reduction in the numbers of pods and seeds and in their dry weight on per plant basis for the tested asparagus bean cultivar, Kao-hsiung green pod.
|Appears in Collections:||1.台灣農業研究(1950～迄今)|
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