|Title:||玉米子粒蛋白質及離氨酸含量測定方法之研究||Other Titles:||Studies on the Methods for Determining Protein and Lysine Content of Corn Kernels||Authors:||鄭心嫻
|Issue Date:||7-Sep-1974||Publisher:||農業試驗所||Journal Volume:||23||Journal Issue:||3||Start page/Pages:||212-217||Source:||農業研究||Abstract:||
The purpose of this study was to compare the rapid method for measuring protein and lysine content of corn kernels, Dye-binding and TNBS vs. Kjeldahl and automatic amino acid analyzer we are using respectively in order to facilitate the analysis of protein and lysine in our high protein and high lysine corn breeding program.
The results indicated that the Dye-binding method for rapid estimation of protein was almost equal to the protein content obtained by Kjeldahl method. (10.17% and 10.20%, See Table 1). The correlation coefficient was 0.857 for protein content obtained by two methods from 54 different opaque-2 inbred lines. One technician could analyze 132 samples per day by using dye-binding method, hut it was only 10 samples by Kjeldahl method.
The lysine content for opaque-2 corn estimated by TNBS method was relatively close to that of the A. A. A. method. However lysine content estimated by this method for floury-2 corn was much lower. This would be due to the different nature of protein for O2 & fl2 corn. The TNBS could analyze 20 samples per day which was much repid than A. A. A. method.
It is suggested that Dye-binding method for protein estimation and TNBS method for lysine determination of O2 corn can be used in corn breeding program.
|Appears in Collections:||1.台灣農業研究(1950～迄今)|
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