低溫處理對水稻花藥接種適期之影響

Abstract

水稻稻穗經不同時問之低溫(10℃)處理後，選取含四分子期至晚單核期小孢子之花藥進行培養，探討低溫處理對花藥接種適期之影響。結果發現，各時期小孢子對低溫處理的反應差異極大。經七天之低溫處理，以中單核期小孢子之花藥最易形成癒合組織，且其誘導率較經低溫處理一天者提高一倍左右。低溫處理14天，可提高晚單核期小孢子之花藥形成癒合組織之能力，但對中單核期之花藥癒合組織誘導率則無提高之效果，過長之低溫處理（超過21天）反會抑制癒合組織之產生。The base of rice panicles with microspores at different developmental stages were wrapped with moist tissue paper and kept at 10℃ for various durations to test the effect of cold shock on callus formation, organ regeneration and chromosome distribution among regenerated plantlets in rice anther culture. Experimental results indicated that culture efficiency of anther increased significantly from cold shock pretreatment. For treatment over two weeks, anther activity could be maintained longer when the moist tissue paper was changed periodically.