不同廠牌及種類之膠體化物質對水稻花藥培養之影響

Abstract

將水稻花藥培養於五種不同品牌之agar時，結果顯示以Difco-Bacto agar (No. 01 40-01)對水稻花藥癒合組織的形成及分化較優，而惠光出品之agar表現最差。A series of experiments were taken to compare the effects of five agar brands (Sigma, Difco-Bacto No. 0140-01, Difco-Bitek No. 0138-01-4, BBL and Huey-Guang) and different gelling agents and concentrations on callus induction and plant regeneration in rice anther culture. In the comparison of the five agar brands, the best results on callus and plant differentiation was obtained with Difco-Bacto agars while the addition of Huey-Guang agar to the medium resulted in the lowest differentiation rate.