|Title:||不同季節生長下母株菸草花葯培養植物體之形成||Other Titles:||Tobacco Plants Formation from Anther Culture as Influenced by the Mother Plants Grown under Different Season||Authors:||蔡新聲
|Issue Date:||9-Jun-1980||Publisher:||農業試驗所||Journal Volume:||29||Journal Issue:||2||Start page/Pages:||147-155||Source:||中華農業研究||Abstract:||
The anthers of N. tabacum cv. Wisconsin 38 which were grown in Hoagland nutrient solution with different nitrogen levels were collected during the period of May-November. The collected anthers were cultured in Murashige and Skoog medium (1962) to produce haploid plants.
It was found that the embryogenic ability of anthers collected from the plants grown under the high temperature and long photoperiod condition (August-September) was extremely lower than those collected from the plants grown under the lower temperature and shorter photoperiod condition. In the hot summer season, the abnormal cells instead of functional microspores in pollen sacs as it was observed by the cytological study. The effects of high temperature and long photoperiod on the abnormal formation of microspore were considered to be the main reason why the embryogenic ability is so poor when this kinds of anthers were cultured in vitro.
Further, it was observed that the rate of abnormal anther was promoted when tobacco plants were cultured under either excess or deficient nitrogen supply condition. The low nitrogen supply resulted in fewer anther formation per flower bud and more anther formation per flower bud under the high nitrogen supply condition as compared with controls.
|Appears in Collections:||1.台灣農業研究(1950～迄今)|
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