異戊烯轉移酶(ipt)基因轉殖青花菜花粉活性及其相近菜種雜交之研究

Abstract

基因流佈 (gene flow) 是基因轉殖作物生態風險評估中一個重要指標，而花粉活性與雜交稔實率是影響作物異交潛力的主要因子。本試驗利用異戊烯轉移酶(isopentenyltransferase, ipt) 基因轉殖青花菜 (Brassica oleracea var. italica) 103 品系與非轉殖青花菜對照組 (104 品系及品種‘綠王’)，和另外5 種相近菜種 [白花芥藍 (B. oleracea var. alboglabra)-‘富悅’、黃花芥藍 (B. oleracea var. alboglabra)-‘白格林’、花椰菜 (B. oleracea var. botrytis)-‘麗雪’、小白菜 (B. rapa var. chinensis)、小油菜 (B. rapa var. japonica)] 等材料，透過分析花粉活性與雜交率對青花菜異交潛力進行評估，提供基因轉殖青花菜商業栽培需隔離與建立共存之參考。 Gene flow is an important index for environmental biosafety assessments. Pollens are potential carriers for genetically modified DNA to other plants. For phanerogams, pollen viability and cross-compatibility are key factors for successful outcross hybridization. In this study, broccoli (Brassica oleracea var. italica) transformed with the ipt gene, designated as line 103 was used to investigate its potential outcross by pollen viability test and pollination with non-transgenic broccolis (line 104 and cv. 'Green King') and closely 5 related varieties, such as Chinese kales (B. oleracea var. alboglabra) 'Fu Yue' (white flower) and 'Bai Ge Lin' (yellow flower), cauliflower (B. oleracea var. botrytis) 'Li Syue', Pakchoi (B. rapa var. chinensis), and edible rape (B. rapa).