台灣產二種藥用石斛的栽培介質及礦物元素分析

Abstract

黃花石斛的幼苗培養於全量MS培養基，添加1.5%蔗糖，不添加任何有機添加物或分別添加8%香蕉泥、8%馬鈴薯汁液或8%椰子汁等四種培養基培養20週，再以水苔或水苔＋中細蛇木屑為栽培介質，移植20週後植株成活率可達93-100%；而僅以中細蛇木屑不含水苔為栽培介質的成活率僅40-67%。 Shi-hu (Dendrobium sp.) is one of the important plants for the traditional Chinese medicine. Dendrobium tosaense and D. moniliforme are the two important native species in Taiwan and they are used commercially as herbs. Seedlings from germinated seeds of D. tosaense transferred onto MS basic salt medium supplemented with 1.5% sucrose, 0.9% Difco agar and 8% banana homogenate or potato juice or coconut water developed into healthy plantlets after culturing for 20 weeks. The 20-week-old plantlets were further transplanted to different culture media (sphagnum moss or tree fern or a mixture of sphagnum moss and tree fern) for ex vivo acclimation. Results showed that 93-100% of the plantlets survived either in sphagnum moss alone or in a mixture of sphagnum moss and tree fern.