Complete Nucleotide Sequence of RNA2 of Cucurbit chlorotic yellows virus Isolated from Melons in Taiwan and Its Comparisons with Currently Existing Isolates

Abstract

The nucleotides of genomic RNA2 of Cucurbit chlorotic yellows virus (CCYV) isolates CCYV-Erlun (JN126045) and CCYV-Yilan (JN126046) collected from Taiwan were completely sequenced. In addition to 5'- and 3'- terminal untranslated regions, a total of 8041 bp in length encoding genes of p4.9 (132 nt), HSP70h (1671 nt), p6 (165 nt), p59 (1554 nt), p9 (240 nt), CP (753 nt), CPm (1425 nt), and p26 (642 nt) were determined by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of 5' complementary DNA ends (5'-RACE). The CP gene-targeted primers for RT-PCR would be useful for improving the sensitivity and accuracy of CCYV detection. 瓜類退綠黃化病毒（Cucurbit chlorotic yellows virus, CCYV） 雲林縣二崙鄉分離株 （CCYV-Erlun）與宜蘭縣壯圍鄉分離株（CCYV-Yilan），以反轉錄聚合酶鏈式反應（RT-PCR）及Rapid amplificationof 5' complementary DNA ends（5' RACE）等方法將其RNA2完全解序，各得全長為8041 bp之核苷酸序列；除 5'- 和 3'- 未轉譯端外，包括p4.9基因（132 nt）、HSP70h基因 （1671 nt）、p6 基因（165nt）、p59基因（1554 nt）、p9基因（240 nt）、CP基因（753 nt）、CPm基因（1425 nt）及p26基因（642nt）。