分子標幟輔助耐鹽水稻之選育

Abstract

本研究以稉型稻耐鹽品系SM61為貢獻親，而世界栽培面積廣泛的優良秈稻品種IR64為輪迴親，透過回交方式將SM61之耐鹽性轉移至IR64，過程中利用200 mM氯化鈉(NaCl)溶液的鹽分逆境處理挑選出耐鹽的植株，並於各回交世代F1進行分子標幟輔助背景選拔，結果發現各BC1F1、BC2F1及BC3F1世代回復輪迴親比率分別為75.0、89.0、96.4%，高於傳統回交方式之BC3F1世代回復至輪迴親之93.8%的結果，顯示利用分子標幟輔助可較快速回復輪迴親IR64之遺傳背景，提高耐鹽水稻新品種之選育效率。 The salt-tolerant mutant line 'SM61' was crossed to IR64, an elite indica variety which is widely grown in the world, to introgress gene(s) conferring salt tolerance to 'IR64' as the recurrent parent by backcrossing. The progenies of each backcross generation were screened salt tolerance by the treatment of 200 mM NaCl and consequently selected by markers for background selection to recover the recurrent parent's genome. The means of recurrent parent's genome were 75.0, 89.0 and 96.4% in selected BC1F1, BC2F1 and BC3F1 individuals, respectively.