https://scholars.tari.gov.tw/handle/123456789/14051| Title: | 新型綠螢光基因AcGFP 及ZsGFP 在水稻之表現、評估與應用 | Other Titles: | Expression, Evaluation and Application of Novel Green Fluorescent Protein Genes, AcGFP and ZsGFP, in Rice | Authors: | 王怡雯 賴慧真 謝曉鶯 王強生 林大鈞 Yi-Wen Wang Hui-Chen Lai Hsiao-Ying Hsieh Chang-Sheng Wang Da-Gin Lin |
Keywords: | 綠螢光基因;報導基因;啟動子;表現系統;農桿菌媒介轉形;水稻;Green fluorescent protein gene;Reporter;Promoter;Expression system;Agrobacteriummediated transformation;Rice | Issue Date: | 7-Dec-2014 | Publisher: | 農業試驗所 | Journal Volume: | 63 | Journal Issue: | 4 | Start page/Pages: | 307-319 | Source: | 台灣農業研究 | Abstract: | 高效率、無害的基因表現篩選系統為所有作物基因轉殖必須克服的問題，爰此本研究建構新型＂AcGFP＂及＂ZsGFP＂報導基因表現系統， 並於水稻進行基因功能評估。首先完成pCAMBIA35SAcGFP及pCAMBIA35SZsGFP表現載體之構築，利用農桿菌媒介轉形法，將螢光基因導入水稻基因組。 The aim of this study is to construct novel expression system driving reporter genes, ＂AcGFP＂ and ＂ZsGFP＂, for functional evaluation of rice genes. Two constructs, pCAMBIA35S＂AcGFP＂ and pCAMBIA35S＂ZsGFP＂, were completed and then introduced into rice genomes by ＂Agrobacterium＂-mediated transformation. |
URI: | https://scholars.tari.gov.tw/handle/123456789/14051 | ISSN: | 0022-4847 | DOI: | 10.6156/JTAR/2014.06304.07 |
| Appears in Collections: | 1.台灣農業研究(1950～迄今) |
| File | Size | Format | |
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| 臺灣農業研究63(4)-7.pdf | 4.17 MB | Adobe PDF | View/Open |
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