https://scholars.tari.gov.tw/handle/123456789/14062| Title: | 利用綠螢光標定Xanthomonas oryzae pv. oryzae偵測水稻感病過程 | Other Titles: | Development of an Efficient Fluorescent Visualization Method for the Detection of Xanthomonas oryzae pv. oryzae Infection in Rice Plants | Authors: | 林大鈞 林玉鈴 陳純葳 賴慧真 王怡雯 周思儀 王強生 Da-Gin Lin Yu-Ling Lin Chun-Wei Chen Hui-Chen Lai Yi-Wen Wang Szu-Yi Chou Chang-Sheng Wang |
Keywords: | 白葉枯病菌;兔海葵綠螢光基因;Xanthomonas 螢光表現系統;病勢調查;病原菌-宿主間交互作用;Xanthomonas oryzae pv. oryzae;ZsGFP gene;Xanthomonas fluorescent expression system;Investigation of disease progress;Pathogen-host interaction | Issue Date: | 2-Mar-2015 | Publisher: | 農業試驗所 | Journal Volume: | 64 | Journal Issue: | 1 | Start page/Pages: | 10-20 | Source: | 台灣農業研究 | Abstract: | 為能即時觀察白葉枯病菌於剪葉接種後感染水稻的完整過程，本研究利用新型強效的ZsGFP基因及pBBR1MCS載體，構築可於白葉枯病病原菌Xanthomonas中表現綠色螢光之重組質體pBRRZsGFP。接著，轉型至Escherichia coli DH5α及Xanthomonas oryzae pv. oryzae XF89b。 Detection of pathogen infection is an important step in pathogen-host interaction study. A Xanthomonas fluorescent expression plasmid, pRBBZsGFP, was constructed with a strong fluorescent gene ZsGFP and the pBBR1MCS vector for immediate detection of bacterial blight pathogen infection. The recombinant plasmid was transformed into Escherichia coli DH5α and Xanthomonas oryzae pv. oryzae XF89b, respectively. |
URI: | https://scholars.tari.gov.tw/handle/123456789/14062 | ISBN: | 0022-4847 | DOI: | 10.6156/JTAR/2015.06401.02 |
| Appears in Collections: | 1.台灣農業研究(1950～迄今) |
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| 64-1-2.pdf | 3.25 MB | Adobe PDF | View/Open |
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