地黃無菌播種及微體繁殖研究

Abstract

本研究利用地黃（Rehmannia glutinosa Libosch）種子進行無菌播種，地黃種子以0.6%次氯酸鈉溶液消毒15 min可獲得良好之殺菌效果，可明顯降低汙染率，並有最高發芽率，分別為5%與80%。以無菌播種地黃小苗之葉片與葉柄作為微體繁殖材料，接種於含有3%蔗糖、0.8% Bacto agar及0.01 mg L-1 NAA之MS培養基（以下簡稱基本培養基）並添加0－2.0 mg L－1 BA不同濃度，分別於光照與黑暗環境下培養。 In vitro studieswere conducted to establish protocol for micropropagation of Rehmannia glutinosa Libosch, an important Chinese herbal medicine. Results showed that seeds disinfection with 0.6% sodium hypochlorite for 15 min significantly reduced the contamination rate to 5% and obtained the highest germination rate to 80%.