透氣處理對香蕉組織培養苗生長、馴化與氣孔發育之影響

Abstract

香蕉組培苗需要馴化後才能適應外界環境。在本試驗中組培瓶封口方式改以不織布材質取代鋁箔，以增加瓶內頂部空間的透氣效率，4種透氣處理分別為：不透氣6 wk（hermetically sealed for 6 weeks; S6V0）、不透氣4 wk後透氣2 wk（4 weeks sealed followed by 2 weeks ventilation; S4V2）、不透氣2 wk後透氣4 wk（2 weekssealed followed by 4 weeks ventilation; S2V4）及透氣6 wk（ventilation for 6 weeks; S0V6）。 Micropropagated banana (＂Musa＂ spp. 'Formosa') requires discret management to acclimatize to the external environment. In this study, aluminum foil was replaced by non-woven fabric to improve ventilation efficiency of culture vessel headspace air condition. Different ventilation treatments of culture vessel were investigated, including hermetically sealed for 6 weeks (S6V0), 4 weeks sealed followed by 2 weeks ventilation (S4V2), 2 weeks sealed followed by 4 weeks ventilation (S2V4), and ventilation for 6 weeks (S0V6). No ethylene accumulation was detected in S4V2, S2V4, and S0V6 treatments; however, high ethylene level (203.3 nL L-1) under airtight vessels of S6V0 was noticed and promoted adventitious root formation on banana plantlets.