Cymbidium ringspot virus 核酸及血清檢測法之建立及其應用

Abstract

Cymbidium ringspot virus (CymRSV) 為國外已有發生之病毒，目前國內尚未有此病毒之發生紀錄。本研究針對此病毒進行其核酸及血清檢測試劑製備，以應用於蘭花病毒監測。核酸檢測法方面，以對應CymRSV 鞘蛋白 (coat protein; CP) 之核苷酸序列設計2 組引子對，應用於反轉錄-聚合酶鏈鎖反應法 (reverse transcription-polymerase chain reaction; RT-PCR)。 Cymbidium ringspot virus (CymRSV) is recorded in foreign countries, but not found in Taiwan. The molecular and serological detection reagents for the inspection of CymRSV on orchids were developed in this study. In molecular detection, two primer pairs for reverse transcription-polymerase chain reaction (RT-PCR) were designed based on the nucleotide sequences of CymRSV coat protein (CP).