https://scholars.tari.gov.tw/handle/123456789/14343
Title: | Optimization of somatic embryogenesis in suspension cultures of horsegram [Macrotyloma uniflorum (Lam.) Verdc.]—A hardy grain legume | Authors: | Shamsudeen Varisai Mohameda Jih-Min Sung Toong-Long Jeng Chang-Sheng Wang |
Keywords: | embryogenic callus;cell suspension culture;somatic embryos;immature cotyledon;Macrotyloma uniflorum | Issue Date: | Oct-2005 | Publisher: | Elsevier | Journal Volume: | 106 | Journal Issue: | 3 | Start page/Pages: | 427-439 | Source: | Scientia Horticulturae | Abstract: | Cell suspension cultures were established from immature cotyledon derived calli from drought tolerant legume horsegram [Macrotyloma uniflorum (Lam.) Verdc.]. Embryogenic callus could be originated from cut slices of the immature cotyledons on MS solid medium [Murashige, T. Skoog, K., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497] augmented with 1.0 μM zeatin and 4.5 μM NAA. Numerous somatic embryoids (26.4%) appeared on MS liquid basal nutrient medium with 5.6 μM NAA and with absence of zeatin during 3 weeks culture. Sustained cell division resulted in the formation of cell aggregates, and then progressed to globular, heart and further if they differentiate properly to torpedo and cotyledonary stages within 5 weeks. Transfer of individual embryos on to a fresh MS basal medium with no plant growth regulators was able to achieve complete maturation. Only a relatively few number of embryos developed into root/shoot when transferred to 0.9 μM GA3, 15 g/l−1 sucrose and 2.4 g/l−1 gelrite containing medium. Substitution of sucrose associated with the use of l-glutamine gave, in the range of concentrations tested, the strongest enhancement of the embryo growth and development. About 5% of somatic embryos were converted into true-to-type fertile plants. |
URI: | https://scholars.tari.gov.tw/handle/123456789/14343 | ISSN: | 0304-4238 | DOI: | 10.1016/j.scienta.2005.05.005 |
Appears in Collections: | SCI期刊 |
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