| DC Field | Value | Language |
|---|
| dc.contributor | Division of Biotechnology, Taiwan Agricultural Research Institute | en |
| dc.contributor.author | Cheng-Ping Kuan | en_US |
| dc.contributor.author | Wen-Shi Chang | en_US |
| dc.contributor.author | Tso-Chi Yang | en_US |
| dc.creator | Cheng-Ping Kuan | en |
| dc.creator | Wen-Shi Chang | en |
| dc.creator | Tso-Chi Yang | en |
| dc.date | 2018-11 | - |
| dc.date.accessioned | 2018-12-14T02:18:43Z | - |
| dc.date.accessioned | 2020-05-12T08:58:55Z | - |
| dc.date.available | 2018-12-14T02:18:43Z | - |
| dc.date.available | 2020-05-12T08:58:55Z | - |
| dc.date.issued | 2018-11 | - |
| dc.identifier.issn | 0191-2917 | - |
| dc.identifier.uri | https://scholars.tari.gov.tw/handle/123456789/14383 | - |
| dc.description.abstract | In this study, we describe multiplex polymerase chain reaction (PCR) coupled with the LiquiChip assay for the identification of Zucchini yellow mosaic virus, Cucumber green mottle mosaic virus, and Cucumber mosaic virus by coamplification with plant mRNA as an internal control. Multiplex reverse-transcription (RT)-PCR products were subjected to allele-specific primer extension, then hybridized to carboxylated microspheres with unique fluorescent identifiers followed by detection using the LiquiChip 200 workstation. This assay is highly specific for distinguishing individual viruses from a mixed viral population and is 10 times more sensitive than multiplex RT-PCR. In addition, the establishment of this method enabled the detection of cucurbit viruses in field samples. | en_US |
| dc.format.extent | 130 bytes | - |
| dc.format.mimetype | text/html | - |
| dc.language | en_US | en |
| dc.language.iso | en_US | en_US |
| dc.publisher | American Phytopathological Society | en_US |
| dc.relation.ispartof | Plant Disease | en_US |
| dc.title | Use of Fluorescent Microsphere-Based Assay for Detection of Three Cucurbit-Infecting Viruses | en_US |
| dc.type | journal article | en_US |
| dc.identifier.doi | 10.1094/PDIS-09-17-1355-RE | - |
| dc.identifier.pmid | 30221583 | - |
| dc.identifier.isi | 000447818800031 | - |
| dc.relation.journalvolume | 102 | en_US |
| dc.relation.journalissue | 11 | en_US |
| dc.relation.pages | 2324-2329 | en_US |
| item.fulltext | with fulltext | - |
| item.cerifentitytype | Publications | - |
| item.languageiso639-1 | en_US | - |
| item.openairetype | journal article | - |
| item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
| item.grantfulltext | open | - |
| crisitem.author.dept | Biosafety Laboratory | - |
| crisitem.author.dept | Biotecnology Division | - |
| crisitem.author.parentorg | Biotecnology Division | - |
| crisitem.author.parentorg | Taiwan Agricultural Research Institute, Council of Agriculture, Executive Yuan | - |
| Appears in Collections: | SCI期刊
|
