Molecular and Morpho-agronomic Characterization of NaN3-induced Common Bean Mutants

Abstract

In this study, amplified fragment length polymorphism (AFLP) and morpho-agronomic diversities in Hwachia, a common bean (Phaseolus vulgaris L.) variety, and its 34 NaN3-induced mutants (M6 generation) were investigated. Eight primer combinations generated 482 fragments from the tested materials, of which 402 fragments were polymorphic. The calculated Jaccard similarity coefficients based on AFLP data ranged from 0.469 to 0.839. Both clustering and principal coordinate analyses confirmed the presence of molecular diversities among Hwachia and its mutants. Morpho-agronomic variations were also observed among the tested mutants. The relations between Hwachia and its mutants based on AFLP data were correlated with those based on morpho-agronomic characteristics (r = –0.713**). Thus, NaN3-induced mutagenesis indeed broadened the genetic variation, and possibly produced new varieties in the common bean. Mutants SA-07, SA-08, SA-09, SA-14, SA-30 and SA-31 are useful as sources for deriving new varieties. 本研究利用擴增增殖片段長度多型性及形態與農藝性狀之差異來探討菜豆品種花莢種及其34 個疊氮化鈉誘變突變系之遺傳變異。所使用八個引子對在供試突變系共產生482 個DNA 片段，其中402 個DNA 片段為多形性片段。依據擴增增殖片段長度多型性資料所估算之Jaccard 相似性係數介於0.469 與0.839 之間。群叢及主軸分析確認花莢種及其34 個疊氮化鈉誘變突變系的確有明顯的分子層次變異。供試材料間也有明顯的形態與農藝性狀差異。此二種特性也有極顯著的相關性 (r =0.713**)。因此，疊氮化鈉引起的誘變的確擴大遺傳變異，並可能產生新的菜豆品種。其中突變系SA-07，SA-08，SA-09，SA-14，SA-30 及SA-31 可做為育成新品種之材枓。