https://scholars.tari.gov.tw/handle/123456789/1534
Title: | Establishment of a Rice Mutant Library for Functional Genomics | Authors: | Yue-Ie Hsing Chyr-Guan Chen Ming-Jen Fan Sheng-Chung Huang Po-Chang Lu Ku-Ting Chen Shuen-Fang Lo Peng-Kai Sun Shin-Lon Ho Kuo-Wei Lee Yi-Chieh Wang Wang-Lii Huang Swee-Suak Ko Shu Chen Jyh-Long Chen Chun-I Chung Yao-Cheng Lin Ai-Ling Hour Yet-Walt Wang Ya-Chi Chang Min-Wei Tsai Yi-Show Lin Yin-Chin Chen Hsing-Mu Yen Charng-Pei Li Chiu-Kai Wey Ching-Shan Tsen Ming-Hsing Lai Liang-Jwu Chen Su-May Yu |
Issue Date: | Dec-2006 | Publisher: | 農業試驗所 | Related Publication(s): | 農業試驗所特刊第126號 | Start page/Pages: | 61-72 | Source: | Ecological and Environmental Biosafety of Transgenic Plants | Conference: | Proceedings of International Symposium Ecological and Environmental Biosafety of Transgenic Plants | Abstract: | Rice has been a model monocot for functional genomics research due to its small genome size (430 Mb) relative to other cereals, its ease of transformation, and its economic importance. With the completion of genomic sequencing analysis, the next challenge will be to assign functions of 37,000 predicted rice genes. During years 2002 to 2006, we have generated nearly 60,000 gene knockout or activation transgenic rice mutant lines using a T - DNA insertional mutagenesis approach. These mutant lines provide important resources for functional analysis of cereal genes using both forward and reverse genetics approaches. The forward genetics approach would screen for mutants with abnormal phenotypes grown under various conditions or treatments, and the reverse genetics approach would identify mutants based on information of flanking sequence tag (FST). By applying these approaches, currently, we have identified many mutants with altered phenotypes of important agronomic traits. Functions of genes responsible for these phenotypes are being analyzed. Novel genes with essential functions will be employed for cereal crop improvement through genetic engineering approaches. |
URI: | https://scholars.tari.gov.tw/handle/123456789/1534 | ISBN: | 978-986-00-7734-6 986-00-7734-7 |
Appears in Collections: | 作物種原組 |
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publication_no126-8.pdf | 3.3 MB | Adobe PDF | View/Open |
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